Product;Sample Type; Description;Number of reactions; Bar Code

DNA Extraction Kits from Tissue specimens "Tissue", Raissol®; Tissue; This DNA extraction method from animal tissues is the classical method with total alcohol precipitation of nucleic acids with several improvements: improved tissue lysis and added the enzyme RNase to degrade RNAs; 50/250; #tissue-50 #tissue-250 DNA Extraction Kits from Tissue specimens "Tissue M", Raissol®; Tissue;This DNA extraction method from animal tissues by sorption on magnetic particles makes it possible to obtain high concentrations of DNA for subsequent applications without the risk of nucleic acids loss during the washing stages, which is typical for classical methods of alcohol precipitation; 50/250; #tissueM-50 #tissueM-250 DNA Extraction Kits from epithelial cells scrapings, smears, cell washes of saliva and urine "Epi Q", Raissol®; Epithelial Cells, Smears, Cell Washes; This DNA extraction method from scrapings of epithelial cells, smears, and cell washes, based on the sorption of nucleic acids on the silicon membrane of spin columns, is a fast and convenient method for extracting DNA. Specially developed reagents make it possible to carry out high-quality lysis in a short time and increase the efficiency of DNA sorption on the silicon membrane of spin columns ;50/250 ; #epiQ-50 #epiQ-250 Nucleic Acids Extraction Kits from PCR mixtures and/or agarose gel fragments "Amplex", Raissol®;PCR mixture, agarose gel fragments; A method for purifying nucleic acids from PCR mixtures and agarose gel fragments, based on the sorption of nucleic acids on the silicon membrane of a spin column. Specifically developed binding reagent allows you to dissolve agarose gels of any percentage, and the optimal salt content and pH level stimulate the sorption of DNA on the silicon membrane;50/250; #amplex-50 #amplex-250 Nucleic Acids Extraction Kits from FFPE specimens "FFPE MAG", Raissol®; FFPE blocks;DNA extraction from sections of FFPE blocks by sorption on magnetic particles is a universal solution that eliminates the harmful effects of toxic components of classical dewaxing methods. Deparaffinization is carried out with a non-toxic, non-volatile solution, which can be removed for ease of use and does not require washing of the fixed tissue. A specially developed lysis solution removes protein-DNA cross-links without a “boiling” step, i.e. does not require temperatures above 85℃, and, as a result, does not lead to the production of denatured DNA samples - the resulting DNA is suitable for preparing libraries using the "ShotGun Sequensing" technology; 50/250; #FFPEMAG-50 #FFPEMAG-250 Metagenome DNA Extraction Kits from feces "Meta Fec", Raissol®; Feces; Meta Fec is a kit, part of the Meta product line from Raissol®, designed specifically for the extraction of metagenomic DNA from feces samples, without requiring expensive equipment and without complex surgical procedures. Extraction step takes 80 minutes, and sorption step on magnetic particles makes it possible to reduce DNA losses in comparison to column sorption methods. The multicomponent lysis solution allows you to isolate DNA from both Gram-positive and Gram-negative bacteria, as well as yeast and yeast-like fungi. The isolated nucleic acids are suitable for any further applications, including PCR, restriction digestion, preparation of whole genome libraries, etc.; 50; #metafec-50 DNA Extraction Kits from bone and dental tissue samples “Bone M”, Raissol®; Bone;The Kit for DNA extraction from bone and dental tissue samples Bone M is a specially developed Raissol® kit that does not require an additional decalcification step, which is present in most NA isolation kits for such group of samples. Sorption of NA on magnetic particles minimizes the risk of losing a small amount NC, characteristic of the methods of classical alcohol precipitation and sorption on silicon membranes of spin columns; 50; #boneM-50; DNA Extraction Kits from seeds, flour and sprouts “SeedExt”, Raissol®; Plant Seeds; The SeedExt ™Raissol reagent Kit is an excellent solution for the DNA extraction from seeds and flour, allowing you to quickly and efficiently obtain high-quality DNA, avoiding the influence of secondary plant metabolites, such as phenols, polysaccharides, etc. For working with seedlings, we recommend using the homogenization buffer included in the SeedExt + kit option, with which homogenization can be easily carried out in a mortar and pestle, or in automatic homogenizers. The resulting nucleic acid samples are suitable for any enzymatic applications, incl. PCR, restriction, etc.;50/250; #SeedExt-50 #SeedExt-250 DNA Extraction Kits from seeds, flour and sprouts “SeedExt+”, Raissol®;Plant Seeds;The SeedExt ™Raissol reagent Kit is an excellent solution for the isolation of DNA from seeds and flour, allowing you to quickly and efficiently obtain high-quality DNA, avoiding the influence of secondary plant metabolites, such as phenols, polysaccharides, etc. For working with seedlings, we recommend using the homogenization buffer included in the SeedExt + kit option, with which homogenization can be easily carried out in a mortar and pestle, or in automatic homogenizers. The resulting nucleic acid samples are suitable for any enzymatic applications, incl. PCR, restriction, etc.; 50/250; #SeedExt+-50 #SeedExt+-250 Yeast Nucleic Acid Extraction Kits "Yeaxt", Raissol®; Yeast;This extraction method of nucleic acids from yeast combines 3 buffers for destruction of the cell wall and total lysis, followed by sorption of nucleic acids on a magnetic sorbent. Excess RNA can be removed using RNase A during the lysis step, or using the Yeaxt+ kit, which includes the optional Raissol® RNase A component; 50;#yeaxt-50; Yeast Nucleic Acid Extraction Kits "Yeaxt+", Raissol®; Yeast; A method for isolating nucleic acids from yeast, based on a combination of 3 buffers for destruction of the cell wall and total lysis, followed by sorption of nucleic acids on a magnetic sorbent. The kit includes the enzyme RNase A RaissolTM, which degrades excess RNA (characteristic of yeast in the growth stage); 50;#yeaxt+_50; Plasmid DNA Extraction Kits from the bacterial culture "Plasmidex Maxi", Raissol®;Bacteria;Plasmid DNA extraction Kit from E. coli cell culture.; 25/50; #plasmidexmaxi-25 #plasmidexmaxi-50; Plasmid DNA Extraction Kits from the bacterial culture "Plasmidex Midi", Raissol® ;Bacteria; Plasmid DNA extraction Kit from E. coli cell culture.;25/50; #plasmidexmidi-25 #plasmidexmidi-50 Plasmid DNA Extraction Kits from the bacterial culture "Plasmidex Mini", Raissol® ; Bacteria; Plasmid DNA extraction Kit from E. coli cell culture; 50/250;#plasmidexmini-50 #plasmidexmini-250 DNA Extraction Kits from complex tissue specimens, Raissol®; Tissue; DNA extraction kits from complex biopsy samples and alcohol preparations of animal tissues, incl. arthropods, etc. A specially designed lysis allows the release of DNA even from denatured protein structures, but requires increased lysis time, and sorption of NC on magnetic particles allows minimizing the risk of nucleic acids loss at the washing stages, which is inherent in classical methods of nucleic acids precipitation; 50 ; #tissue_strong-50 Metagenome DNA Extraction Kits from soil specimens, Raissol®; Soil; Meta Soil is a kit, part of the Meta product line from Raissol®, designed specifically for the extraction of metagenomic DNA from soil samples, without requiring expensive equipment and without complex operational procedures. Isolation of one sample takes no more than 80 minutes, which is several times superior to classical isolation methods, and sorption on magnetic particles makes it possible to reduce NC losses compared to column sorption methods. The multicomponent lysis solution allows the isolation of DNA from both gram+ and gram- bacteria, including the spore stage, as well as yeast cells. The isolated nucleic acids are suitable for any further applications, including PCR, restriction digestion, preparation of whole genome libraries, etc.; 50; #metasoil-50; Nucleic Acids Extraction Kits from milk "Meta Milk", Raissol®; Milk; Meta Milk is a kit, part of the Meta product line from Raissol®, designed specifically for the extraction of metagenomic DNA from small volume milk specimens (up to 500 µl), which does not require expensive equipment and does not include complex operational procedures. Extraction step takes 80 minutes, and sorption step on magnetic particles makes it possible to reduce NC losses in comparison with column sorption methods. The multicomponent lysis solution allows the isolation of DNA from both Gram-positive and Gram-negative bacteria, as well as yeast and yeast-like fungi. The isolated nucleic acids are suitable for any further applications, including PCR, restriction digestion, preparation of whole genome libraries, etc.; 50 ; #metamilk-50 Nucleic Acids Extraction Kits from milk "Meta Milk Maxi", Raissol®; Milk; Meta Milk is a kit , part of the Meta product line from Raissol®, designed specifically for the extraction of metagenomic DNA from large volume milk samples (up to 3000 µl), which does not require expensive equipment and does not include complex operational procedures. Extraction step takes 80 minutes, and sorption step on magnetic particles makes it possible to reduce NC losses in comparison with column sorption methods. The multicomponent lysis solution allows the isolation of DNA from both gram+ and gram- bacteria, as well as yeast and yeast-like fungi. The isolated nucleic acids are suitable for any further applications, including PCR, restriction digestion, preparation of whole genome libraries, etc.; 25/50; #metamilk_maxi-25 #metamilk_maxi-50 RNA Extraction Kits by sorption on spin columns “RNA Pro Q”, Raissol®;Tissues; The Kit of reagents is intended for the RNA extraction from animal and plant tissues, mammalian cell cultures, bacteria, and yeast; ;#RNAproQ_test #RNAproQ-50

Product; Conc range; Description;Bar Code

Kits of reagents for DNA concentration measurement with the Qubit “Spectra Q HS Plus” fluorimeter, Raissol®; 0.1 - 100 ng/μl;The Spectra Q HS Plus Raissol™ reagent kit is designed to measure the concentration of double-stranded DNA in solutions in the range of 0.1 - 100 ng/µl;#spectraHS1000+ Kits of reagents for measuring DNA concentration on the Qubit “Spectra Q BR Plus” fluorimeter, Raissol®; 2 – 1000 ng/µ;The Spectra Q BR Plus Raissol™ reagent kit is designed to measure the concentration of double-stranded DNA in solutions in the range of 2 – 1000 ng/µl;#spectraBR1000+

Product;Compatibility; Description;Number of reactions; Bar Code

Library Preparation Kits from amplicons “SG GM Ampli”, Raissol®; Illumina/GeneMind; A kit for libraries preparation from amplicons up to 600bp for sequencing on the Illumina platform without a fragmentation step allows you to obtain high-quality libraries in a short time. A simple repair step followed by ligation without the need for DNA cleanup between steps saves the user time and minimizes the possibility of error and cross-contamination. Indexing is carried out using PCR - which is compatible with indexed primers using Illumina TrueSeq technology. 6 cycles of PCR indexing are recommended, but changes are allowed depending on the required final DNA concentration and the relative number of duplicates. Purification after the indexing step is recommended to be carried out using magnetic particles using SPRI technology (it is important to take into account the manufacturer’s recommendations on the number of particles added per required size of fragmented DNA). ;96/192; SG_GM_ampli-96 #SG_GM_ampli-192 Comprehensive NGS libraries Preparation Kits «SG GM Plus», Raissol®;Illumina/GeneMind;The SG_GM reagent kit from Raissol® is a complete system for enzymatic library preparation using ShotGun Sequensing technology - simple manipulations allow you to obtain fragmented DNA in the range of 130bp from genomic DNA or amplicon DNA with a length of more than 800bp. Size regulation can be achieved by changing the time of the fragmentation stage, and to process fragmented DNA there is no need to purify it after the fragmentation stage. The repair and ligation stages are carried out sequentially in one tube. Purification after the ligation step is performed using specially designed Raissol® particles using SPRI technology without the need to wash and dry the particles, which significantly reduces library preparation time. Indexing is carried out using PCR - the system is compatible with indexed primers using Illumina TrueSeq technology. 8 cycles of PCR indexing are recommended, but changes are allowed depending on the required final DNA concentration and the relative number of duplicates.Cleaning after the indexing stage is carried out with specially developed Raissol® particles using SPRI technology. ; 96/192; #SG_GM_plus-96 #SG_GM_plus-192 Comprehensive Library Preparation Kits (larger than 300 bp) "SG GM Maxi" , Raissol®;Illumina/GeneMind;The reagent kit is intended for the preparation of whole-genome libraries larger than 300bp using enzymatic fragmentation of genomic DNA.; 96/192; #SG_GM_maxi-96 #SG_GM_maxi-19 Comprehensive Library Preparation Kits (larger than 300 bp) "SG GM Maxi Plus", Raissol®;Illumina/GeneMind;The reagent kit is intended for the preparation of whole-genome libraries larger than 300 bp using enzymatic fragmentation of genomic DNA.; 96/192; #SG_GM_maxi_plus-96 # SG_GM_maxi_plus-192 A plate with indexed primers of the first set for the preparation of comprehensive libraries for Illumina platform in a dual indexing system, Raissol®;Illumina/GeneMind;Indexed primers are compatible with the following kits: SG GM, SG GM Maxi, SG GM Plus, SG GM Ampli, SG GM Maxi Plus; ; #Plate1_SG_GM A plate with indexed primers of the second set for the preparation of comprehensive libraries for Illumina platform in a dual indexing system, Raissol®;Illumina/GeneMind;Indexed primers are compatible with the following kits: SG GM, SG GM Maxi, SG GM Plus, SG GM Ampli, SG GM Maxi Plus; ;#Plate2_SG_GM

Product;Description;Bar code

Taq Ready Red buffer 2.5x, Raissol®;A DNA sample and primers must be added. 100 reactions (25µl). 1x Taq Ready Red buffer contains Tris pH=8.9, Mg2+ 2.5 мМ, dNTP 0.25 мМ.Ready PCR buffer for sample loading “Taq Red” should be stored at -20°C for 12 months from the manufacturer’s release date;#Taqrb2.5x SD Red, Raissol®; A DNA sample and primers must be added. 100 reactions (25µl).1x SD Ready Red buffer contains Tris-HCl pH=8.9 65 мМ, Mg2+ 2.5 мМ, dNTP 0.25 мМ. Store the prepared PCR buffer for adding samples “SD Ready Red buffer 2.5x” at -20°C for 12 months from the manufacturer’s release date;#SDrb2.5x Short Step Marker, Raissol; Pre-mixed with loading buffer. The 10 and 50 bp fragments are present in increased concentrations to facilitate their identification in the gel. Visualization of the DNA marker is possible by staining with intercalating dyes: ethidium bromide, SYBR Gold, SYBR Safe, etc. Can be stored at temperatures as low as +4℃ when in use. In case of long-term storage, a temperature of -16°C to -20°C must be maintained for max 12 months from the manufacturer’s release date; #ssm Middle Step Marker, Raissol®; Pre-mixed with loading buffer. The 500 bp fragments are present in increased concentrations to facilitate their identification in the gel. Visualization of the DNA marker is possible by staining with intercalating dyes: ethidium bromide, SYBR Gold, SYBR Safe, etc. Can be stored at temperatures as low as +4℃ when in use. In case of long-term storage, a temperature of -16°C to -20°C must be maintained for max 12 months from the manufacturer’s release date; #msm; Blue Buffer, Raissol®; The 6x buffer contains two dyes. In a 1.5% agarose gel, the electrophoretic mobility of the blue dye corresponds to DNA fragments of ~ 1000 bp, the blue dye migrates at a speed of ~ 100 bp. The buffer is intended for use in TBE/TAE agarose gels. Suitable for DNA fragments from 100 bp. up to 3000 bp. Store at temperatures from -20℃ to +20℃ for 12 months from the manufacturer's release date; #blb; Green Buffer, Raissol®; The 6x buffer contains two dyes. In a 1.5% agarose gel, the electrophoretic mobility of the blue dye corresponds to DNA fragments of ~ 1000 bp, the orange dye migrates at a speed of ~ 50 bp. The buffer is intended for use in TBE/TAE agarose gels. Suitable for DNA fragments from 50 bp. up to 3000 bp. Store at temperatures from -20℃ to +20℃ for 12 months from the manufacturer's release date; #glb Orange Buffer, Raissol®; The 6x buffer contains two dyes. In a 1.5% agarose gel, the electrophoretic mobility of the yellow dye corresponds to DNA fragments of ~500 bp. The orange dye migrates at a speed of ~50 bp.The buffer is intended for use in TBE/TAE agarose gels. Suitable for DNA fragments from 50 bp. up to 500 bp. Store at temperatures from -20℃ to +20℃ for 12 months from the manufacturer's release date; #olb; Nucleic asids co-precipitator for extraction, Raissol®; The co-precipitant is linear polyacrylamide. It is used to increase the efficiency of nucleic acid precipitation with ethanol and isopropanol, especially when working with solutions with low concentrations of DNA and RNA. Facilitates the visualization of the resulting NC precipitate, which avoids losses of the target product during deposition. It is effective for precipitation of both short NAs (from 20 bases) and long NAs molecules. It does not affect the quality and shelf life of DNA and RNA. It does not affect various enzymatic reactions (amplification, restriction, ligation, etc.). Concentration 5 mg/ml. Recommended volume: 50 µg per 500 µl of product. Packaging 500µl/2.5 mg Store at temperatures from +2℃ to +8℃ for 12 months from the manufacturer's release date;#sat; 5x Taq buffer K+, Raissol®; 1000µl 5x buffer. Contains Mg2+, dNTP. 1x Taq buffer K+ contains Tris pH=8.9, Mg2+ 2.5 mM, dNTP 0.25 mM. Store at -20°C for 12 months from the date of manufacture ;#taqK 5x Taq buffer NH+4, Raissol®; 1000µl 5x buffer. Contains Mg2+, dNTP. 1x Taq buffer NH4+ contains Tris pH=8.9, Mg2+ 2.5 mM, dNTP 0.25 mM. Store at -20°C.; #taqNH4; Taq HS, Raissol®; Taq Hot Start DNA polymerase is a recombinant Taq DNA polymerase protein inactivated by monoclonal antibodies (5µg/µL). Antibodies block polymerase activity at temperatures below 75 C. Activation of the enzyme occurs during primary denaturation for at least 1 minute. Operating temperature: 70-74 C; #Taqpol-1000, #Taqpol-5000, #Taqpol-10000; SD HS, Raissol®; SD Hot Start DNA polymerase is a mutant thermostable polymerase inactivated by monoclonal antibodies (10µg/µL). Antibodies block polymerase activity at temperatures below 75 C. Activation of the enzyme occurs during primary denaturation for at least 1 minute.Operating temperature: 68 C. SD polymerase is effective for template amplification with low concentration of short (from 100 bp) and long (up to 20-30 kb) fragments, allows you to overcome the secondary structures of primers and templates; #SDpol-1000/#SDpol-5000/#SDpol-10000; Phusion R, Raissol®; Phusion R Hot Start DNA polymerase (1µg/µL) is a derivative of Pfu polymerase with an additional Sso7d domain, inactivated by monoclonal antibodies. Enzyme activation occurs during primary denaturation for at least 1 minute. Operating temperature: 72 C.Phusion R DNA polymerase is effectively suitable for amplification of long DNA sections (up to 30 thousand bp), high-precision amplification of DNA fragments for cloning. Provides high accuracy and speed of amplification;#PR-100 #PR-200 #PR-500; Optima PCR-buffer for SD-polymerase «SD buffer optima 2.5x», Raissol; Optimal 2.5x PCR buffer for SD and SD HS DNA polymerases. The buffer is optimized for efficient and reproducible PCR, including real-time PCR with intercalating dyes. SD buffer optima 2.5x contains components that increase the processivity of SD polymerase during PCR. 1x "SD optima buffer" contains Tris pH=8.9, Mg2+ 2.5 mM, dNTP 0.25 mM. Store at temperatures from -20℃ to -16℃ for 12 months from the manufacturer's release date ; #SDoptima Reagent for PCR products purification “ExON”, Raissol;The ExON reagent allows purification of the PCR product in one step and is added to the product after the end of the amplification process, followed by two incubations: the first at a temperature of 37℃ to cleave residual primers and dephosphorylate nucleotides. The second – at a temperature of 80℃ to inactivate the reagent. Samples treated with the ExON reagent do not require additional purification and are ready for research immediately after completion of the processing process. If necessary, it is recommended to carry out a desalting step of the product to enhance the fluorescence signal. Operating temperature: 37℃. Inactivation temperature: 80℃.; #ExON-0.5 #ExON-1 #ExON-4 Kits for whole genome amplification “WGA Classic”, Raissol®; The kit is developed for the whole genome amplification procedure. Store at temperatures from -20℃ for 12 months from the manufacturer's release date;#wga_classic-50 #wga_classic-100; Set of reagents for DNA and RNA decontamination “TermiNAtor”, Raissol;TermiNAtor is a highly effective two-component set of reagents for treating work surfaces and equipment for the purpose of decontamination from nucleic acids. The operating principle is based on the chemical degradation of the contaminant. The kit is effective against both genomic DNA and RNA molecules and PCR amplicons. The kit is suitable for regular cleaning of laboratory work areas to maintain a high level of cleanliness, as well as in cases of unintentional contamination with concentrated nucleic acid solutions.Components: 1 Lysis buffer, 2 R-reagent, 3 Neutralizing buffer, 4 PCR buffer, 5 Polymerase, 6 Buffer for dilution. Efficacy was confirmed by specific real-time PCR.; #termiNAtor