Next generation sequencing (NGS) is one of the key technologies for genome analysis. This method uses modern technology to obtain a large amount of gene sequence information. Sequencing NGS has a number of advantages, including high speed, a wide range of applications in medical diagnostics, and the ability to use various methods for processing genomic information and identifying mutations. One of the most popular NGS sequencing systems is Illumina, which provides high accuracy and quality results. Illumina can be used to determine the complete genome sequences of humans and other organisms. The NGS sequencing process is based on the parallel determination of the sequences of DNA or RNA fragments in a sample. Includes the stages of sample preparation, cluster synthesis, read acquisition and analysis of the obtained data. NGS sequencing offers rapid and accurate detection of mutations in genes, which is an important tool in molecular medicine. It also has a wide range of applications in the clinical diagnosis of various diseases and the creation of individual solutions for patients. NGS sequencing is an affordable and efficient method, synthesizing a large number of nucleotides simultaneously. It also ensures data privacy and security during analysis. There are several laboratories and companies in Russia that offer NGS sequencing services using modern technologies and affordable prices. Due to this, a large number of research works and the development of new projects are based on the use of next-generation sequencing. SesanaGenomics has 17 years of practical experience in sequencing for a variety of applications. We have our own functioning laboratory with all the products presented in stock and in active use in Russia, Central Asia, Australia and Partners in China. We invite you for a tour and training on all NGS equipment and reagents.»
SURFSeq 5000 High-throughput Sequencing Platform from GeneMind
SURFSeq 5000 is a high-throughput, whole-genome sequencing instrument. All library sample preparation protocols used on Illumina platforms are already adapted for SURFSeq 5000. The function of 6 options for generating FastQ data in real time has appeared for the first time on the NGS market! This innovation provides the ability to output 6 FastQ files during sequencing for fast analysis. This provides greater flexibility compared to similar devices from other manufacturers. A new approach to sequencing - running flow cells in parallel! If, while sequencing your first flow flow cell, you have another batch of samples ready for sequencing, there's no need to wait! Easily install a second flow cell and begin sequencing while the first step is still in progress. This process, what we call “parallel running,” sets a new standard for efficient sequencing workflow. The operation of the device is based on the principle of sequencing by synthesis (SBS) Each flow cell can be loaded independently, so two lanes on a cartridge can run in parallel with different samples or sequencing types, reducing instrument runtime and expanding the range of applications within a single experiment. Comprehensive -8 human genomes per launch Multitasking -2 independent flow cells with the ability to manually load libraries Continuous sequencing -Starting a new flow cell before the first run is completed Flexible -Allows to select the number of sequencing cycles before data release 8 human genomes per launch -2 independent flow cells Manual and Automatic loading of libraries -Automatic wash Productive and fast: 1200 GB in 48 hours Up to 2000M reads per launch
GenoLab M High-throughput Sequencing Platform from GeneMind
Genolab M is a new generation DNA sequencer based on modern technologies. With this device, you can quickly and accurately obtain information about the DNA structure. One of the main features of Genolab M is its high performance - it is able to process a large number of DNA fragments in a short time. Various methods are used to obtain the DNA sequence, including chemical reactions and electrophoresis. Genolab M differs from other sequencers with ability to determine both short and long DNA sequences, including modifications and mutations. This is essential for clinical studies and diagnosis of individual cases. The Genolab M sequencer is used in a variety of applications in biology and genomic medicine. It allows you to obtain information about the molecular structure of cells and other samples, as well as detect mutations and other errors. One of the most important features of Genolab M is the information processing program, which allows you to automate the process of obtaining and analyzing data. This device also has a wide range of capabilities, including the ability to handle small volumes of materials and carry out complex technical tasks. The operation of the device is based on the principle of sequencing by synthesis (SBS). Each flow cell can be loaded independently, so two lanes on a cartridge can run in parallel with different samples or sequencing types, reducing instrument runtime and expanding the range of applications within a single experiment. The Genolab M sequencer is based on modern technologies and ensures high accuracy and confidentiality of data. The cost of the device may be even less than that of the first known sequencers, making it accessible to a wide group of scientists and medical professionals. The platform characteristics are similar to the popular Illumina NextSeq 2000 platform*
*Lamshchikov P. et al. Comparison of the Illumina NextSeq 2000 and GeneMind Genolab M sequencing platforms for spatial transcriptomics. BMC Genomics. (2023). 24(1):102. DOI: 10.1186/s12864-023-09192-w. PMID: 36882687; PMCID: PMC9990361. * Li C et al. Accuracy benchmark of the GeneMind GenoLab M sequencing platform for WGS and WES analysis. BMC Genomics. (2022). 23(1):533. DOI: 10.1186/s12864-022-08775-3. PMID: 35869426; PMCID: PMC9308344. *Liu Y et al. Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis. BMC Genomics. (2021). 22(1):829. DOI: 10.1186/s12864-021-08150-8. Erratum in: BMC Genomics. 2022 Jan 26;23(1):81. PMID: 34789158; PMCID: PMC8600837.
FASTASeq 300 High-throughput Sequencing Platform
Highest sequencing speed: Thanks to implemented advanced chemistry, and simultaneous signal reading and chemical reaction, FASTASeq 300 produces results in a record time - SE50 in 4.5 hours - PE75 in 11.5 hours - PE150 in 19.5 hours Wide range of multiplex capabilities FCM and FCH flow cells for 100 and 250 million reads have 4 independent lanes, which allows you to increase the number of samples in one run by 4 times TruSeq/Nextera compatible: `Prepare libraries with any TruSeq/Nextera-based kits SURF-seqTM - Sequencing is carried out using 4 nucleotides labeled with different fluorophores, the 3′ end of which is blocked (reversible terminators).
Each flow cell can be loaded independently of the other, so two lanes on a cartridge can run in parallel with different samples or sequencing types, reducing instrument runtime and expanding the range of applications within a single experiment.
NGS Library Prep/Nucleic acids extraction
Opentrons OT-2
OT-2 can automate hundreds of routine protocols and workflows Fast, neat and accurate: Allows you to obtain consistently accurate results with any number of technical replicates. User-friendly software: A simple graphical interface allows you to create and launch your own protocols in a few minutes. Compatible with any laboratory equipment: The Opentrons brand includes laboratory equipment, designed and tested with maximum precision. If you already have lab equipment from other brands, you may be able to use them. Automatic Nucleic Acid Extraction System BK-HS96, Biobase System for the extraction of nucleic acids on paramagnetic particles - 11-60 minutes per highlight round - extraction from 1-96 samples per round - sample volume 20-1000 µl - UV disinfection and HDPE filter - Heating module up to 105◦ C, with an accuracy of 0.1◦ C
Automatic Nucleic Acid Extraction System BK-AutoHS96, Biobase
System for nucleic acids extraction and preparation of PCR mixtures - fully automated stages of sampling + nucleic acids extraction (on magnetic particles) + adding reagents for PCR - up to 60 minutes to prepare 96 samples per round - up to 3 rounds per launch - sample volume 20-1000 µl - UV disinfection and HEPA filter - Heating module up to 105◦ C, with an accuracy of 0.1◦ C
Auto Nucleic Acid Extraction System BNP96, Biobase
System for DNA and RNA extraction on magnetic particles - suitable for the extraction of nucleic acids from blood, tissues, throat swabs, etc., without centrifugation or filtration - 15-40 minutes per highlight round - extraction from 1-96 samples per round - sample volume 20-100 µl - product purity (A260/A280): DNA > 1.7-2.0; RNA > 1.8-2.1 - UV disinfection - Heating module up to 120◦ C, with an accuracy of 0.1◦ C Nucleic Acid Extraction System BNP32 / BNP48, Biobase System for Nucleic Acid Extraction on magnetic particles: - 15-40 minutes per highlight round - allocation of 32/48 samples per round - sample volume 20-500 µl - UV disinfection - Built-in heating module - Built-in control system - no need to connect to a PC DNA & RNA Auto Nucleic Acid Purification Extraction System BK-HS32 Compact solution for DNA and RNA extraction: - 15-60 minutes per highlight round - extraction of 32 samples per round - sample volume 20-1000 µl - UV disinfection, HEPA filter - Built-in heating module - Built-in control system - no need to connect to a PC
PCR equipment
PCR Gradient Thermo Cyclers, PCRTC-G, Bioevopeak
Gradient type Capacity: 96-well plate, 96X0.2 ml PCR tubes Maximum heating/cooling speed: 5℃/sec. Temperature uniformity≤±0.3℃ 7" large color touch screen
Mini Thermal Cycler, PCRTC-16, Bioevopeak
Ultra-light and ultra-thin Capacity: 16 0.2 ml PCR tubes Maximum heating/cooling speed: 4℃/sec. Temperature uniformity≤±0.2℃ Temperature range: 4~99.9℃ Hot cap:30-110℃
Standard PCR Machine, PCRTC-S, Bioevopeak
No gradient heating Capacity: 96-well plate, 96X0.2 ml PCR tubes Maximum heating/cooling speed: 5℃/sec. Temperature uniformity≤±0.3℃ 7" large color touch screen
PCR-96A Gradient Thermal Cycler, Bioevopeak
6 independent temperature zones Optimized Gradient Function Capacity: 96X0.2 ml PCR tubes Maximum heating/cooling speed: 5℃/s Temperature uniformity ≤±0.2℃ Maximum number of cycles: 100
PCR-96S Gradient Thermal Cycler, Bioevopeak
Capacity: 96X0.2 ml PCR tubes Maximum heating/cooling speed: 4℃/s Temperature uniformity≤±0.2℃
PCR-602S Gradient Thermal Cycler, Bioevopeak
High throughput capacity: 384-well plate Maximum heating/cooling speed: 4.5℃/s Temperature uniformity≤±0.2℃ Reaction volume: 10-30 µl Temperature range: 0-105℃
PCR-482 Gradient Thermal Cycler, Bioevopeak
Independently controlled 4 temperature zones Capacity: double 48×0.2 ml Maximum heating/cooling speed: 5℃/s, 4℃/s Temperature uniformity≤±0.2℃
PCR-G96XG Gradient Thermal Cycler, Bioevopeak
Capacity: 3× (32×0.2ml) Large memory capacity and USB device support Color touch screen Email notification function Maximum heating/cooling speed: 5℃/s, 4℃/s Temperature uniformity≤±0.3℃
Real-time PCR equipment
Real-time PCR System, PCR-Q96-5, Bioevopeak
Capacity: 96 wells Luminescent channels: 5/6 Maximum heating/cooling speed: 6.0°C/s
Real-time PCR System, PCR-Q96-6, Bioevopeak
Capacity: 96 wells Luminescent channels: 6/6 Maximum heating/cooling speed: 6.0°C/s