The data included in our reports for every sequence are Phred20 analysis, *.ABI and *.scf trace files and seq files in text format. Verification sequencing reports provide the following: insertions, deletions, mismatches between reference sequence and sequencing data, and alignment between reference sequence and sequencing data.

Sanger Sequencing and Fragment Analysis

Sanger sequencing is used to study a small subset of genes linked to a defined phenotype, confirm next-generation sequencing (NGS) variants, detect minor allele fractions down to 5%, or read contiguous sequences up to 1,000 bases.

Fragment analysis is a powerful technique with simple, straightforward workflows and is used in a wide-range of applications, such as detection of mutations, genotyping, identification of short tandem repeats, and gene expression profiling.
We use Applied Biosystems genetic analysis systems - a trusted standard for Sanger sequencing and fragment analysis by capillary electrophoresis.