Reagent kit for DNA extraction from whole blood "GM Blood Q" according to TU 20.59.52-005-95224908-2021 Raissol

Raissol

Reagent kit for DNA extraction from whole blood "GM Blood Q" according to TU 20.59.52-005-95224908-2021 Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with krov.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;DNA extraction from blood;Agrogenetics>>>Agrogenetics - nucleic acid extraction;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typeKrov

Sample Type: Krov

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. Lysis buffer - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
2. Binding buffer - dlya povysheniya sorbtsii NA na filtre kolonki;
3. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
4. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
6. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
7. Spin-kolonki - dlya filtratsii reaktsionnoy smesi i sorbtsii NA.

V nabor reagentov "GM Blood Q" variant 240R vklyucheny sleduyushchie raskhodnye materialy:
1.Odnorazovye nakonechniki 1000 uL;
2.Odnorazovye nakonechniki 200 uL;
3.Adaptery rotora dlya stantsii QIAcube.

Storage conditions

Buffers nabora "GM Blood Q" can be stored pri temperature ot +4 do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Enzyme Proteinase K must be stored pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) fermenta Proteinase K ot +4℃ do +25℃ for no more than 5 days.

Required materials and equipment

Neobkhodimye reagenty, materialy i oborudovanie dlya "GM Blood Q" variant 50 i variant 250:
  1. Ethanol 96%;
  2. Microcentrifuge tubes obemom 1,5-2 mL;
  3. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  4. Variable-volume pipettes 20-200 uL, 100-1000 uL;
  5. Odnorazovye nakonechniki dlya dozatorov peremennogo obema s aerozolnym barerom do 200 uL i 1000uL;
  6. Mernyy tsilindr obemom 100 mL s tsenoy deleniya 1 mL;
  7. Vorteks;
  8. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone ot +25 do +80ºC dlya probirok obemom 1,5-2 mL;
  9. High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min.
Neobkhodimye reagenty, materialy i oborudovanie dlya "GM Blood Q" variant 240R:
  1. Ethanol 96%;
  2. Microcentrifuge tubes obemom 1,5-2 mL;
  3. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  4. Avtomaticheskiy dozator peremennogo obema 100-1000 uL;
  5. Odnorazovye nakonechniki dlya dozatorov peremennogo obema s aerozolnym barerom do 1000uL;
  6. Mernyy tsilindr obemom 100 mL s tsenoy deleniya 1 mL;
  7. Avtomaticheskaya stantsiya QIAcube Connect (Qiagen, Germaniya);
  8. Reagent Bottles 30 ml (6 sht.) Cat. No.: 990393 (Qiagen, Germaniya).