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Nucleic acid extraction kit from yeast "Yeaxt+" Raissol

Raissol
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Nucleic acid extraction kit from yeast "Yeaxt+" Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with drozhzhi.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typeDrozhzhi

Sample Type: Drozhzhi

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. White bufer - dlya resuspendirovaniya issleduemogo obraztsa;
2. Blue bufer - na osnove ionnykh PAV i auxiliary components dlya kletochnogo lizisa;
3. Yellow bufer - na osnove chaotropic agents i auxiliary components dlya polnogo lizisa;
4. Binding buffer - dlya povysheniya sorbtsii NA na magnitnykh chastitsakh;
5. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
6. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
7. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
8. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
9. RNase A - dlya udaleniya RNA;
10. Magnitnye chastitsy - dlya sorbtsii NA.

Instructions for use

DNA extraction procedure

Lysis
1. V tubes obemom 1,5-2 mL place 100 uL White bufera i perenesti proby (razmerom so spichechnuyu golovku) s tverdoy pitatelnoy sredy sterilnym instrumentom. V sluchae zhidkoy pitatelnoy sredy, tsentrifugirovat samples pri 8-13 tys. ob/min i polnostyu udalit supernatant - kolichestvo osadka ne dolzhno prevyshat razmer spichechnoy golovki - i resuspendirovat konglomerat kletok v 100 uL White bufera.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Podgotovit neobkhodimyy obem reagenta iz rascheta 100 uL Blue bufera i 10 uL RNAazy A na 1 reaktsiyu (naprimer, 500 uL Blue bufera i 50 uL RNAazy A na 5 reactions).
4. Vnesti 110 uL podgotovlennogo reagenta v kazhdyy sample, mix thoroughly i inkubirovat at room temperature v techenie 3-5 minut, peremeshivaya samples kazhduyu minutu.
5. Vnesti v samples 200 uL Yellow bufera, mix thoroughly i add 20 uL Proteinazy K.
6. Incubate tubes v termostate pri 56-60℃ v techenie 30 minut, periodicheski vstryakhivaya.
7. Centrifuge tubes v techenie 3 minut pri 8-13 tys. ob/min. Perenesti supernatant v novye tubes obemom 1,5-2 mL, ne zakhvatyvaya pellet.
Sorbtsiya NA
  1. Add k smesi 200 uL svyazyvayushchego bufera i 50 uL magnitnykh chastits.
  2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
  3. Incubate samples at room temperature v techenie 5 minut, periodicheski peremeshivaya.
  4. Sbrosit kapli s pomoshchyu brief centrifugation i ustanovit tubes na magnitnyy shtativ. Incubate v techenie 3-5 minut.
  5. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
Nucleic acid washing
1. Add v tubes 500 uL promyvochnogo bufera 1.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate na magnitnom shtative v techenie 1-2 minut, akkuratno, ne zadevaya chastitsy, udalit supernatant.
4. Add v tubes 500 uL promyvochnogo bufera 2.
5. Repeat punkty 2-3.
6. Vysushit samples s otkrytymi kryshkami v termostate pri 56-60℃ v techenie 10-15 minut until complete ispareniya promyvochnogo rastvora.
Nucleic acid elution
1. Add v tubes 50-100 uL elyuiruyushchego bufera.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate v termostate pri 56-60℃ v techenie 5-10 minut, periodicheski peremeshivaya.
4. Cbrosit kapli s pomoshchyu brief centrifugation.
5. Incubate na magnitnom shtative v techenie 2-3 minut.
6. Perenesti elyuat v novye tubes obemom 1,5-2 mL.
Optsionalno: rekomenduetsya provesti dopolnitelnoe tsentrifugirovanie elyuata na maksimalnoy skorosti dlya udaleniya ostatochnogo kolichestva magnitnykh chastits.
Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Po absorbance ratio na A260/A280 purity of the resulting solution DNA corresponds to ≥1,7.

Download the short instruction "Yeaxt+" (pdf)

Download the full instruction "Yeaxt+" (pdf)

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Storage conditions

Buffers nabora "Yeaxt+" can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Magnitnye chastitsy can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. Pered applicationm suspenziyu chastits neobkhodimo tshchatelno vzbaltyvat do odnorodnogo sostoyaniya.
Enzyme Proteinase K must be stored pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) fermenta Proteinase K ot +4℃ do +25℃ for no more than 5 days.
Enzyme RNase A khranitsya pri -20℃, shelf life is 12 mesyatsev.

Required materials and equipment

  1. Microcentrifuge tubes obemom 1,5-2 mL;
  2. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  3. Variable-volume pipettes na 2-20 uL, 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
  4. Vorteks;
  5. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL;
  6. Magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL;
  7. High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min.