SD Hot Start DNA polymerase "SD HS" 10U/uL Raissol
Raissol
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SD Hot Start DNA polymerase "SD HS" 10U/uL Raissol is a Raissol reagent product for pcr workflow support in molecular biology, genetics, diagnostic and research laboratories.
Application area
Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.
Sample types
The product is intended for work with biological material.
Advantages of Raissol reagents
stable reagent performance and reproducible laboratory results
compatibility with common molecular biology protocols
convenient workflow for routine and research procedures
efficient preparation of biological material for downstream analysis
Key specifications
Category
PCR reagents and supplies;Raissol
Manufacturer
Raissol
Primary use
PCR workflow support
Sample type
biological material
Instructions for use
Prigotovlenie reaktsionnoy smesi
Programma PCR
Storage conditions
Instructions for use
SD Hot Start DNA-polimeraza predstavlyaet soboy mutantnuyu termostabilnuyu polimerazu, inaktivirovannuyu monoklonalnymi antitelami. Antitela blokiruyut polimeraznuyu aktivnost pri temperature do 75 C. Aktivatsiya fermenta proiskhodit vo vremya pervichnoy denaturatsii v techenie ne menee 1 minuty. SD-polimeraza effektivno podkhodit dlya amplifikatsii matritsy c nizkoy kontsentratsiey, korotkikh (ot 100 bp) i dlinnykh (do 20-30 t.bp) fragmentov, pozvolyaet preodolevat vtorichnye struktury praymerov i matritsy. Edinitsa aktivnosti (e.a.): kolichestvo fermenta, neobkhodimoe dlya vklyucheniya 10 nmol dNTP v kislotonerastvorimuyu fraktsiyu v techenie 30 minut pri temperature 68 C. Usloviya termotsiklirovaniya: parametry amplifikatsii variruyutsya v zavisimosti ot matritsy, praymerov i komponentov bufernoy smesi.
