DNA extraction kit from animal tissue "Tissue M" Raissol

Raissol

DNA extraction kit from animal tissue "Tissue M" Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with tkani.

Advantages of Raissol reagents

stable reagent performance and reproducible laboratory results
compatibility with common molecular biology protocols
convenient workflow for routine and research procedures
efficient preparation of biological material for downstream analysis

Key specifications

Category	DNA and RNA extraction kits;Agrogenetics>>>Agrogenetics - nucleic acid extraction;Raissol
Manufacturer	Raissol
Primary use	DNA/RNA extraction
Sample type	Tkani

Sample Type: Tkani

Kit contents

Instructions for use

Storage conditions

Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. Lysis buffer 1 - na osnove detergentov i auxiliary components dlya optimal lysis;
2. Lysis buffer 2 - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
3. Binding buffer - dlya povysheniya sorbtsii NA na magnitnykh chastitsakh;
4. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
6. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
7. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
8. Magnitnye chastitsy - dlya sorbtsii NA.

Instructions for use

DNA extraction procedure

Lysis
1. V tubes obemom 1,5-2 mL place issleduemye samples.
2. V sluchae khraneniya obraztsov v fiziologicheskom rastvore tsentrifugirovat samples v techenie 5 minut pri 13 tys. ob/min, posle chego polnostyu udalit supernatant.
3. Add k obraztsam 200 uL liziruyushchego bufera 1, 30 uL Proteinazy K, mix thoroughly on a vortex mixer.
Optsionalno: vozmozhno ispolzovanie RNAazy A lyubogo proizvoditelya v kontsentratsiyakh, rekomendovannykh proizvoditelem.
4. Incubate samples v termostate pri 56℃ v techenie 2-5 chasov, periodicheski vstryakhivaya.
5. Centrifuge samples v techenie 5 minut pri 13 tys. ob/min., posle chego perenesti 200 uL supernatanta v novye tubes obemom 1,5-2 mL.
6. Vnesti 200 uL liziruyushchego bufera 2, peremeshat tubes on a vortex mixer i sbrosit kapli s pomoshchyu brief centrifugation.
Sorbtsiya NA
1. Add k smesi 200 uL svyazyvayushchego bufera i 50 uL magnitnykh chastits, mix thoroughly tubes on a vortex mixer.
2. Incubate v techenie 3-5 minut at room temperature, periodicheski vstryakhivaya.
3. Sbrosit kapli s pomoshchyu brief centrifugation.
4. Ustanovit tubes na magnitnyy shtativ i inkubirovat v techenie 3-5 minut.
5. Akkuratno, ne zadevaya pellet, udalit supernatant.
Nucleic acid washing
1. Add v tubes 700 uL promyvochnogo bufera 1, mix thoroughly tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
2. Incubate na magnitnom shtative v techenie 1-2 minut.
3. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
4. Add v tubes 700 uL promyvochnogo bufera 2, mix thoroughly tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
5. Incubate na magnitnom shtative v techenie 1-2 minut.
6. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
7. Repeat punkty 4-6.
8. Vysushit samples s otkrytymi kryshkami v termostate pri 56-60℃ ili at room temperature until complete ispareniya promyvochnogo rastvora.
Nucleic acid elution
1. Add v tubes 50-100 uL elyuiruyushchego bufera.
2. Incubate v termostate pri 56-60℃ v techenie 5 minut, periodicheski peremeshivaya on a vortex mixer.
3. Sbrosit kapli s pomoshchyu brief centrifugation.
4. Incubate na magnitnom shtative v techenie 1-2 minut.
5. Perenesti elyuat v novye tubes obemom 1,5-2 mL.
Optsionalno: rekomenduetsya provesti dopolnitelnoe tsentrifugirovanie elyuata na maksimalnoy skorosti dlya udaleniya ostatochnogo kolichestva magnitnykh chastits.

Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Po absorbance ratio na A260/A280 purity of the resulting solution DNA sootvetstvuyut ≥1,7.

Download the short instruction "TissueM" (pdf)

Download the full instruction "TissueM" (pdf)

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Storage conditions

Buffers nabora "Tissue M" can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya warm the solutions to room temperature.
Magnitnye chastitsy can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. Pered applicationm suspenziyu chastits neobkhodimo tshchatelno vzbaltyvat do odnorodnogo sostoyaniya.
Enzyme Proteinase K must be stored pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) fermenta Proteinase K ot +4℃ do +25℃ for no more than 5 days.

Required materials and equipment

Microcentrifuge tubes obemom 1,5-2 mL;
Magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL;
Variable-volume pipettes na 2-20 uL, 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
Vorteks;
Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL;
High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min.