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Spectra Q BR kit for quantitative DNA determination (2-1000 ng) Raissol

Raissol
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Spectra Q BR kit for quantitative DNA determination (2-1000 ng) Raissol is a Raissol reagent product for dna/rna quantification in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with biological material.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryNucleic acid and protein quantification;Agrogenetics>>>Agrogenetics - concentration measurement;Raissol
ManufacturerRaissol
Primary useDNA/RNA quantification
Sample typebiological material
Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA quantification:
1. Spectra Q BR dye - interkalyator;
2. Spectra Q BR buffer - bufernyy rastvor;
3. Standard 1 - kalibrator;
4. Standard 2 - kalibrator.

Instructions for use

Double-stranded DNA concentration measurement procedure

1. Prigotovit master-miks na neobkhodimoe kolichestvo reactions iz rascheta:
Spectra Q BR dye -1 uL na 1 reaktsiyu.
Spectra Q BR buffer - 199 uL na 1 reaktsiyu.
2. Prigotovit standarty v tonkostennykh prozrachnykh probirkakh: Standard 1 - 10 uL pervogo standarta razvodyatsya v 190 uL master-miksa; Standard 2 - 10 uL vtorogo standarta razvodyatsya v 190 uL master-miksa.
3. Podgotovit samples: v otdelnuyu tonkostennuyu prozrachnuyu tube vnositsya 200-Vobr uL master-miksa i Vobr uL analiziruemogo obraztsa (variruetsya ot 1 do 10 uL). Naprimer, pri izmerenii proby Vobr=2 uL v tube vnositsya 200-2=198 uL master-miksa i 2 uL izmeryaemoy proby.
4. Vse samples i standarty peremeshat on a vortex mixer. Sbrosit kapli so stenok s pomoshchyu brief centrifugation.
5. Na fluorimetre Qubit vybrat metody "dsDNA".
6. V otkryvshemsya menyu vybrat programmu "Broad Range".
7. Pred izmereniem otkalibrovat pribor, nazhav knopku "Read standards". Poocheredno izmerit snachala 1-y standart, zatem 2-y.
8. Posle zamera oboikh standartov nazhat knopku "Run samples". Ustanovit obem obraztsa, v zavisimosti ot togo, kakoy obem obraztsa vnosilsya v tube.
9. Posledovatelno izmerit kontsentratsii obraztsov.

Vazhno! Standarty dolzhny khranitsya pri temperature ot +2℃ do +8℃. Interkalyator Spectra Q BR dye mozhet zamerzat pri khranenii ot +2℃ do +8℃ i imeet srodstvo k RNA v 10 raz nizhe, chem k DNA.

Download the short instruction "SpectraBR" (pdf)

Download the full instruction "SpectraBR" (pdf)

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Storage conditions

Spectra Q BR buffer must be stored pri temperature ot +4℃ do +25℃ for 12 months c daty vypuska izgotovitelya.
Standard 1 i Standard 2 nabora "Spectra Q BR" dolzhny khranitsya pri temperature ot +2℃ do +8℃ for 12 months, ne dopuskaya zamorazhivaniya. A short-term storage temperature increase is allowed (transportation) ot +4℃ do +25℃ for no more than 5 days.
Interkalyator Spectra Q BR dye must be stored at room temperature v nedostupnom dlya sveta meste for 12 months. Pri dolgosrochnom khranenii interkalyator dolzhen khranitsya pri temperature ot -20℃ do +4℃ v nedostupnom dlya sveta meste for 12 months i bolee.

Required materials and equipment

  1. Fluorometer Qubit (ThermoFisher Scientific, SShA);
  2. Microcentrifuge tubes obemom 1,5-2 mL;
  3. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  4. Prozrachnye tonkostennye tubes obemom 0,5 mL bez graduirovki (naprimer, Qubit™ Assay Tubes, Invitrogen);
  5. Shtativ dlya probirok obemom 0,5 mL;
  6. Variable-volume pipettes na 0,5-10, 2-20 uL, 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
  7. Vorteks.