DNA extraction kit from whole blood Blood E Raissol

The kit includes all reagents required for DNA extraction:
1. Lysis buffer - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
2. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
3. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
4. Wash buffer 3 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Wash buffer 4 - dlya promyvki nucleic acids ot cell metabolites and salts;
6. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
7. Magnitnye chastitsy - dlya sorbtsii NA.

Download the short instruction "GM Blood E" (pdf)

Download the full instruction "GM Blood E" (pdf)

Lysis
1. V tubes obemom 1,5-2 mL place 200 uL krovi i add 400 uL liziruyushchego bufera.
2. Vstryakhnut tubes on a vortex mixer. Sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate v termostate pri 70℃ v techenie 10 minut, periodicheski vstryakhivaya.
4. Incubate at room temperature v techenie 5 min until completely cooled probirok.
Sorbtsiya NA
1. Add v tubes 60 uL magnitnykh chastits.
2. Vstryakhnut tubes on a vortex mixer. Sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate at room temperature v techenie 10 min, periodicheski vstryakhivaya.
4. Sbrosit kapli s pomoshchyu brief centrifugation.
5. Incubate tubes na magnitnom shtative v techenie 5 minut.
6. Akkuratno, ne zadevaya magnitnye chastitsy, udalit supernatant.
Nucleic acid washing
1. Add v tubes 700 uL promyvochnogo bufera 1.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation, inkubirovat na magnitnom shtative v techenie 2 minut.
3. Akkuratno, ne zadevaya magnitnye chastitsy, udalit supernatant.
4. Add v tubes 700 uL promyvochnogo bufera 2.
5. Repeat punkty 2,3.
6. Add v tubes 700 uL promyvochnogo bufera 3.
7. Repeat punkty 2,3.
8. Add v tubes 700 uL promyvochnogo bufera 4.
9. Repeat punkty 2,3.
10. Vysushit samples s otkrytymi kryshkami v termostate pri 56-60℃ v techenie 7 minut, libo at room temperature v techenie 15 minut until complete ispareniya promyvochnogo rastvora.
Nucleic acid elution
1. Add v tubes 50-100 uL elyuiruyushchego bufera.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate v termostate pri 56-60℃ v techenie 5-10 minut, periodicheski peremeshivaya.
4. Sbrosit kapli s pomoshchyu brief centrifugation.
5. Incubate na magnitnom shtative v techenie 2 minut.
6. Perenesti elyuat v novye tubes obemom 1,5-2 mL.
Optsionalno: rekomenduetsya provesti dopolnitelnoe tsentrifugirovanie elyuata na maksimalnoy skorosti dlya udaleniya ostatochnogo kolichestva magnitnykh chastits.
Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Po absorbance ratio na A260/A280 purity of the resulting solution DNA corresponds to ≥1,7.

Buffers nabora "GM Blood E" can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Magnitnye chastitsy can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. Pered applicationm suspenziyu chastits neobkhodimo tshchatelno vzbaltyvat do odnorodnogo sostoyaniya.

Microcentrifuge tubes obemom 1,5-2 mL; shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL; avtomaticheskie dozatory peremennogo obema na 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips; vortex mixer; tverdotelnyy termostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL; magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL; skorostnaya mikrotsentrifuga dlya probirok obemom 1,5-2 mL do 13 tys. ob/min (optsionalno).