DNA extraction kit from epithelial cell scrapes, swabs, saliva cell washes and urine Epi Q Raissol

The kit includes all reagents required for DNA extraction:
1. Lysis buffer - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
2. Binding buffer - dlya povysheniya sorbtsii NA na filtre kolonki;
3. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
4. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
6. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
7. Spin-kolonki - dlya filtratsii reaktsionnoy smesi i sorbtsii NA.

DNA extraction procedure

Lysis
1. V tubes obemom 1,5-2 mL place samples.
2. Add 350 uL liziruyushchego bufera i 20 uL Proteinazy K.
3. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
4. Incubate v termostate pri 55-60℃ v techenie 10-15 minut, periodicheski vstryakhivaya.
Primechanie: vremya mozhet byt uvelicheno do 30 minut dlya povysheniya vykhoda DNA.
5. Ostudit smes at room temperature v techenie 3-5 minut. Perenesti maksimalno vozmozhnyy obem poluchennogo rastvora v novuyu 1,5-2 mL tube.
Nucleic acid sorption and precipitation
1. Add k smesi 350 uL svyazyvayushchego bufera.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Perenesti ves obem smesi v spin-kolonku.
4. Centrifuge tubes v techenie 30 sekund pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
Nucleic acid washing
1. Add v tubes 600 uL promyvochnogo bufera 1.
2. Centrifuge tubes v techenie 30 sekund pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
3. Add v tubes 600 uL promyvochnogo bufera 2.
4. Centrifuge tubes v techenie 30 sekund pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
5. Vernut kolonki v sobiratelnye tubes. Centrifuge tubes v techenie 1 minuty pri maks. ob/min.
Nucleic acid elution
1. Perenesti spin-kolonki v novye tubes obemom 1,5-2 mL. Sobiratelnye tubes utilizirovat.
2. Add na tsentr membrany spin-kolonok 50-100 uL elyuiruyushchego bufera. Incubate v techenie 1-2 minut.
3. Centrifuge tubes v techenie 30 sekund pri maks. ob/min.
4. Utilizirovat spin-kolonki.
Optsionalno: dlya povysheniya vykhoda NA filtrat mozhno povtorno nanesti na membranu kolonki i/ili ispolzovat nagretyy do 60-70℃ elyuiruyushchiy bufer.
Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Po absorbance ratio na A260/A280 purity of the resulting solution DNA sootvetstvuyut ≥1,7.

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Buffers nabora "GM Epi Q" can be stored pri temperature ot +4 do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Enzyme Proteinase K khranitsya pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) ot +4℃ do +25℃ for no more than 5 days.