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DNA cleanup kit after bisulfite conversion "DeSulf Clean" Raissol

Raissol
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DNA cleanup kit after bisulfite conversion "DeSulf Clean" Raissol is a Raissol reagent product for ngs library preparation in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with biological material.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryPCR reagents and supplies;Metilirovanie;Agrogenetics>>>Agrogenetics - library preparation;Raissol
ManufacturerRaissol
Primary useNGS library preparation
Sample typebiological material
Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

V nabor vklyucheny vse neobkhodimye reagenty:
1. Binding buffer - dlya povysheniya sorbtsii NA na filtre kolonki;
2. Desulfiruyushchiy bufer - dlya udaleniya sulfogruppy;
3. Wash buffer - dlya promyvki nucleic acids;
4. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
5. Spin-kolonki - dlya sorbtsii NA.

Instructions for use

Kratkaya instruktsiya "DeSulf Clean" (pdf)

Polnaya instruktsiya "DeSulf Clean" (pdf)

Sorbtsiya NA
1. V spin-kolonki vnesti 3 obema svyazyvayushchego bufera po otnosheniyu k obemu obraztsa.
2. Add ves obem obraztsa posle bisulfitnoy konversii.
3. Mix smes perevorachivaniem kolonok.
4. Centrifuge kolonki v techenie 30 sekund pri 10-12 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
Desulfirovanie i promyvka NA
1. Add v kolonki 200 uL promyvochnogo bufera.
2. Centrifuge kolonki v techenie 30 sekund pri 10-12 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
3. Add v kolonki 200 uL desulfiruyushchego bufera.
4. Incubate kolonki at room temperature 20 minut.
5. Centrifuge kolonki v techenie 30 sekund pri 10-12 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
6. Dvazhdy povtorit punkty 1-2.
7. Centrifuge pustye spin-kolonki v techenie 1 minuty pri 10-12 tys. ob/min.
8. Perenesti spin-kolonki v novye tubes obemom 1,5-2 mL. Sobiratelnye tubes utilizirovat.
Nucleic acid elution
1. Add na tsentr membrany spin-kolonok 22 uL elyuiruyushchego bufera. Incubate v techenie
5 minut at room temperature.
2. Centrifuge kolonki v techenie 1 minuty pri 10-12 tys. ob/min.
3. Utilizirovat spin-kolonki.
Optsionalno: dlya povysheniya vykhoda NA neobkhodimo povtorno nanesti filtrat na membranu kolonki.
Poluchennye rastvory modifitsirovannykh nucleic acids can be stored do 2 dney pri temperature ot +2°C do +4°C i do goda pri temperature -20°C.

Storage conditions

Vse reagenty nabora "DeSulf Clean" can be stored pri temperature ot +4 do +25°C for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.

Required materials and equipment

Microcentrifuge tubes obemom 1,5-2 mL; shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL; avtomaticheskie dozatory peremennogo obema na 20- 200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips; cpin kolonki s sobiratelnymi probirkami (idut v komplekte); vortex mixer; skorostnaya mikrotsentrifuga dlya probirok obemom 1,5-2 mL do 13 tys. ob/min.