SG GM Auto whole-genome library preparation reagent kit for the VNL-96P robotic station with the second index set and magnetic beads Raissol
Raissol
SG_GM_auto-2
SG GM Auto whole-genome library preparation reagent kit for the VNL-96P robotic station with the second index set and magnetic beads Raissol is a Raissol reagent product for ngs library preparation in molecular biology, genetics, diagnostic and research laboratories.
Application area
Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.
Sample types
The product is intended for work with biological material.
Advantages of Raissol reagents
- stable reagent performance and reproducible laboratory results
- compatibility with common molecular biology protocols
- convenient workflow for routine and research procedures
- efficient preparation of biological material for downstream analysis
Key specifications
| Category | Sequencing library preparation kits;Raissol |
| Manufacturer | Raissol |
| Primary use | NGS library preparation |
| Sample type | biological material |
Section: Kity dlya prigotovleniya DNA libraries
1. 3v1 bufer;
2. 3-ferment;
3. Nukleaza Mini;
4. Nukleaza Maxi;
5. Ligaznyy bufer;
6. Ligaza;
7. Adaptery;
8. PCR bufer;
9. PCR ferment 1;
10. PCR ferment 2;
11. Praymery - komplekt indeksov 1 (#SG_GM_auto-1);
12. Praymery - komplekt indeksov 2 (#SG_GM_auto-2);
13. Elution buffer;
14. Magnitnye chastitsy.
2. 3-ferment;
3. Nukleaza Mini;
4. Nukleaza Maxi;
5. Ligaznyy bufer;
6. Ligaza;
7. Adaptery;
8. PCR bufer;
9. PCR ferment 1;
10. PCR ferment 2;
11. Praymery - komplekt indeksov 1 (#SG_GM_auto-1);
12. Praymery - komplekt indeksov 2 (#SG_GM_auto-2);
13. Elution buffer;
14. Magnitnye chastitsy.
Provedenie protsedury prigotovleniya polnogenomnykh libraries
Predvaritelnaya podgotovka biological material
Nukleaza Mini 20 ng/uL (dlya genomnoy DNA) ili 10 ng/uL (dlya amplikonov)
Nukleaza Maxi 30 ng/uL dlya genomnoy DNA
- Vnesti 10 uL obraztsov v 96-lunochnyy planshet (0.2 ml Skirted Micro Well Plates). Kontsentratsiya dolzhna byt ustanovlena:
2) dlya amplikonov - spektrofotometricheski s applicationm interkalyatorov (#spectrahs-100/500/1000; #spectrabr-100/500/1000).
Podgotovka pribora
- Vklyuchit pitanie rabochey stantsii dlya avtomaticheskogo zapuska sistemy (tumbler na pravoy storone pribora).
- Otkryt "WorkStation" na rabochem stole i nazhat v otkryvshemsya okne "Start", posle chego otkroetsya glavnaya stranitsa.
- Nazhat "General Setting", chtoby otkryt interfeys preparation k predvaritelnoy ochistke. Otkryv zashchitnuyu dvertsu, ochistit rabochie poverkhnosti robota 70% etanolom. Zakryt zashchitnuyu dvertsu i nazhat "Pre-cleaning".
- Nazhat "Yes", chtoby vklyuchit blok ventilyatornogo filtra i vypolnit predvaritelnuyu ochistku. Protsess zaymet 20 minut.
- Podgotovit 3 glubokolunochnykh plansheta (1.3 ml Robotic Deep Well Plates):
- pervyy planshet - v kazhduyu lunku vnesti po 100 uL elyuiruyushchego bufera;
- vtoroy planshet - v kazhduyu lunku vnesti po 1 mL 80% etanola, nakryt planshet spetsialnoy kryshkoy;
- tretiy planshet - v kazhduyu lunku vnesti komponenty nabora reagentov v sootvetstvii so skhemoy. Posle vneseniya zakleit planshet folgirovannoy plenkoy i osadit kapli kratkovremennym tsentrifugirovaniem.
2. Osadit kapli kratkovremennym tsentrifugirovaniem v praymerakh - komplekte indeksov 1 ili 2 (v zavisimosti ot komplektatsii).
Zapusk pribora
- Rasstavit v pribor raskhodnye materialy, podgotovlennye planshety s reaktivami i praymery - komplekt indeksov 1 ili 2 (v zavisimosti ot komplektatsii) v sootvetstvii s prilozheniem 1 polnoy versii instruktsii po primeneniyu.
- Udalit vse plenki s planshetov, ostavit tolko kryshku na planshete so etanolom.
- Opustit dvertsu robota i zapustit programmu, predostavlennuyu proizvoditelem. Vypolnenie protsessirovaniya zaymet okolo 3 chasov.
Poluchennye polnogenomnye librariesi can be stored pri +4℃ v techenie sutok ili pri -20℃ bolee dlitelnyy srok. Polnogenomnye librariesi mogut byt ispolzovany v dalneyshey podgotovke k sekvenirovaniyu.