More products

Nucleic acid extraction kit from FFPE blocks "FFPE MAG" Raissol

Raissol
REQUEST NOW

Nucleic acid extraction kit from FFPE blocks "FFPE MAG" Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with ffpe-bloki.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typeFFPE-bloki

Sample Type: FFPE-bloki

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. Start bufer - netoksichnyy, neletuchiy reagent dlya deparafinizatsii;
2. Lysis buffer - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
3. Binding buffer - dlya sorbtsii NA na magnitnykh chastitsakh;
4. Wash buffer 1 - dlya promyvki nucleic acids ot soley;
5. Wash buffer 2 - dlya promyvki nucleic acids ot soley;
6. Wash buffer 3 - dlya promyvki nucleic acids ot soley;
7. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
8. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
9. RNase A - dlya udaleniya RNA;
10. Magnitnye chastitsy - dlya sorbtsii NA.

Instructions for use

DNA extraction procedure

Deparafinizatsiya i lizis
  1. K srezu FFPE-bloka add 600 uL start bufera i 400 uL liziruyushchego bufera, peremeshat samples on a vortex mixer.
  2. Incubate v termostate pri 60℃ v techenie 15 minut until complete dissolution parafina, periodicheski peremeshivaya samples.
  3. Centrifuge samples v techenie 30 sekund, posle chego akkuratno udalit bolshuyu chast verkhney organicheskoy fazy. Rekomenduetsya ostavit tonkiy sloy verkhney fazy vo izbezhanie poteri tkani. Organicheskaya faza ne toksichna i ne vliyaet na dalneyshuyu rabotu nabora.
  4. Add k obraztsam 30 uL Proteinazy K, peremeshat, sbrosit kapli kratkovremennym tsentrifugirovaniem.
  5. Incubate samples pri 60℃ ne menee 3 chasov. Rekomenduetsya inkubirovat samples v techenie nochi dlya polnogo lizisa tkani i bolshego vykhoda NA.
  6. Centrifuge samples pri maks. ob/min v techenie 3 minut, perenesti maksimalnyy obem supernatanta v novye tubes obemom 1,5-2 mL.
  7. Add k obraztsam 5 uL RNAazy A, akkuratno peremeshat, sbrosit kapli kratkovremennym tsentrifugirovaniem.
  8. Incubate samples v techenie 15 minut at room temperature.
Sorbtsiya NA
  1. Add v tubes 600 uL svyazyvayushchego bufera i 60 uL magnitnykh chastits, peremeshat.
  2. Incubate samples v techenie 5 minut at room temperature, periodicheski peremeshivaya.
  3. Sbrosit kapli kratkovremennym tsentrifugirovaniem.
  4. Incubate samples na magnitnom shtative v techenie 2 minut. Akkuratno udalit supernatant, ne zadevaya pellet magnitnykh chastits.
Nucleic acid washing
1. Add v tubes 600 uL promyvochnogo bufera 1.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate tubes na magnitnom shtative v techenie 1 minuty, posle chego akkuratno, ne zadevaya pellet chastits, udalit supernatant.
4. Add v tubes 600 uL promyvochnogo bufera 2.
5. Repeat punkty 2-3.
6. Add v tubes 600 uL promyvochnogo bufera 3.
7. Repeat punkty 2-3.
8. Vysushit samples s otkrytymi kryshkami v termostate v techenie 5-7 minut pri 60℃ until complete ispareniya promyvochnogo bufera 3.
Nucleic acid elution
1. Add v tubes 50-100 uL elyuiruyushchego bufera.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate v termostate pri 60℃ v techenie 10 minut, periodicheski peremeshivaya. Cbrosit kapli s pomoshchyu brief centrifugation.
4. Incubate na magnitnom shtative v techenie 3 minut.
5. Perenesti elyuat v novye tubes obemom 1,5-2 mL.
Optsionalno: rekomenduetsya provesti dopolnitelnoe tsentrifugirovanie elyuata na maksimalnoy skorosti dlya udaleniya ostatochnogo kolichestva magnitnykh chastits.

Poluchennye rastvory nucleic acids can be stored do 4 dney pri temperature ot +2℃ do +4℃ i bolee dlitelnoe vremya pri temperature -20℃.

Download the short instruction "FFPEMAG" (pdf)

Download the full instruction "FFPEMAG" (pdf)

return to product catalog

Storage conditions

Buffers nabora "FFPE MAG" can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Start bufer nabora "FFPE MAG" khranitsya pri temperature ot +20℃ do +25℃ for 12 months from the manufacturer's release date
Magnitnye chastitsy can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. Pered applicationm suspenziyu chastits neobkhodimo tshchatelno vzbaltyvat do odnorodnogo sostoyaniya.
Enzymey Proteinase K i RNase A must be stored pri -20℃, shelf life is 12 mesyatsev.

Required materials and equipment

  1. Microcentrifuge tubes obemom 1,5-2 mL;
  2. Magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL;
  3. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  4. Variable-volume pipettes na 2-20 uL, 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
  5. Vorteks;
  6. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL;
  7. High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 14 tys. ob/min.