More products

DNA quantification reagent kit for Qubit fluorometer "Spectra Q BR Plus" Raissol

Raissol
#spectraBR1000+-5761.0
REQUEST NOW

DNA quantification reagent kit for Qubit fluorometer "Spectra Q BR Plus" Raissol is a Raissol reagent product for dna/rna quantification in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with biological material.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryNucleic acid and protein quantification;Raissol
ManufacturerRaissol
Primary useDNA/RNA quantification
Sample typebiological material
Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA quantification:
1. Spectra Q BR dye - interkalyator;
2. Spectra Q BR buffer - bufernyy rastvor;
3. Standard 1 - kalibrator;
4. Standard 2 - kalibrator;
5. Probirki - tonkostennye polipropilenovye opticheski prozrachnye obemom 0,5 mL.

Instructions for use

Double-stranded DNA concentration measurement procedure

1. Prigotovit master-miks na neobkhodimoe kolichestvo reactions iz rascheta:
Spectra Q BR dye - 1uL na 1 reaktsiyu
Spectra Q BR buffer - 199 uL na 1 reaktsiyu
2. Prigotovit standarty v tonkostennykh prozrachnykh probirkakh: Standard 1 - 10 uL pervogo standarta razvodyatsya v 190 uL master-miksa; Standard 2 - 10 uL vtorogo standarta razvodyatsya v 190 uL master-miksa.
3. Podgotovit samples: v otdelnuyu tonkostennuyu prozrachnuyu tube vnositsya 200-Vobr uL master-miksa i Vobr uL analiziruemogo obraztsa (variruetsya ot 1 do 10 uL). Naprimer, pri izmerenii proby Vobr=2 uL v tube vnositsya 200-2=198 uL master-miksa i 2 uL izmeryaemoy proby.
4. Vse samples i standarty peremeshat on a vortex mixer. Sbrosit kapli so stenok s pomoshchyu brief centrifugation.
5. Na fluorimetre Qubit vybrat metody "dsDNA".
6. V otkryvshemsya menyu vybrat programmu "Broad Range".
7. Pred izmereniem otkalibrovat pribor, nazhav knopku "Read standards". Poocheredno izmerit snachala 1-y standart, zatem 2-y.
8. Posle zamera oboikh standartov nazhat knopku "Run samples". Ustanovit obem obraztsa, v zavisimosti ot togo, kakoy obem obraztsa vnosilsya v tube.
9. Posledovatelno izmerit kontsentratsii obraztsov.

Storage conditions

Spectra Q BR buffer must be stored pri temperature ot +4℃ do +25℃ for 12 months c daty vypuska izgotovitelya.
Standard 1 i Standard 2 nabora "Spectra Q BR" dolzhny khranitsya pri temperature ot +2℃ do +8℃ for 12 months, ne dopuskaya zamorazhivaniya. A short-term storage temperature increase is allowed (transportation) ot +4℃ do +25℃ for no more than 5 days.
Interkalyator Spectra Q BR dye must be stored at room temperature v nedostupnom dlya sveta meste for 12 months. Pri dolgosrochnom khranenii interkalyator dolzhen khranitsya pri temperature ot -20℃ do +4℃ v nedostupnom dlya sveta meste for 12 months i bolee.

Required materials and equipment

Fluorometer Qubit (ThermoFisher Scientific, SShA); mikrotsentrifuzhnye tubes obemom 1,5-2 mL; tubes tonkostennye polipropilenovye opticheski prozrachnye obemom 0,5 mL (vkhodyat v komplekt); shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL; shtativ dlya probirok obemom 0,5 mL; avtomaticheskie dozatory peremennogo obema na 0,5-10, 2-20 uL, 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips; vortex mixer.