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SPRI magnetic beads for selective DNA cleanup "Smart beads" Raissol

Raissol
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SPRI magnetic beads for selective DNA cleanup "Smart beads" Raissol is a Raissol reagent product for ngs library preparation in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with rastvor dna.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;Agrogenetics>>>Agrogenetics - library preparation;Raissol
ManufacturerRaissol
Primary useNGS library preparation
Sample typeRastvor DNA

Sample Type: Rastvor DNA

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

Magnitnye chastitsy "Smart beads" khranyatsya v bufere, optimizirovannom dlya selektivnogo svyazyvaniya opredelennykh fraktsiy fragmentov DNA v zavisimosti ot ispolzuemykh obemov vneseniya magnitnoy suspenzii.

Instructions for use

V khode raboty rekomenduetsya ispolzovat magnitnye chastitsy "Smart beads" komnatnoy temperatury. Neposredstvenno pered ispolzovaniem shake vigorously.
Protsedura selektivnoy cleanup DNA
1. Vnesti obem magnitnykh chastits k obraztsam DNA v sootvetstvii s neobkhodimym razmerom produkta.
2. Intensivno peremeshat on a vortex mixer.
3. Incubate samples at room temperature v techenie 5-10 minut, periodicheski vstryakhivaya.
4. Sbrosit kapli kratkovremennym tsentrifugirovanie i ustanovit samples na magnitnyy shtativ, inkubirovat v techenie 3-5 minut do prozrachnosti rastvora.
5. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
6. Add 80% etanol v obeme ravnom ne menee 2/3 ot obema tubes k kazhdomu obraztsu, inkubirovat v techenie 30 sekund, peremeshchaya tubes na magnitnom shtative, menyaya ikh polozhenie otnositelno magnita. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
7. Repeat punkt 6. Centrifuge sample, ustanovit na magnitnyy shtativ i polnostyu udalit ostatki spirta. Vysushit samples s otkrytymi kryshkami at room temperature ili pri 37℃ until complete ispareniya spirta.
8. Vnesti neobkhodimyy obem elyuiruyushchego bufera (TE-bufera ili deionizovannoy vody). Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
9. Pomestit samples na magnitnyy shtativ, inkubirovat v techenie 2-3 minut do prozrachnosti rastvora.
10. Perenesti neobkhodimoe kolichestvo elyuata v novye tubes, ne zakhvatyvaya magnitnykh chastits.

Poluchennye samples DNA can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Poluchennye samples DNA mogut byt ispolzovany dlya dalneyshikh studies.

Download the short instruction "Smartb" (pdf)

Download the full instruction "Smartb" (pdf)

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Storage conditions

Magnitnye chastitsy "Smart beads" neobkhodimo khranit pri temperature ot + 2℃ do +8℃, shelf life is 12 mesyatsev. Ne zamorazhivat! Razresheno kratkovremennoe khranenie (transportirovka) pri temperature ne vyshe + 25℃ ne bolee 4-kh dney bez poteri obshchego sroka godnosti.

Required materials and equipment

  1. Obraztsy DNA, kotorye neobkhodimo ochistit.
  2. Vorteks.
  3. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS.
  4. Microcentrifuge tubes neobkhodimogo obema.
  5. High-speed microcentrifuge dlya probirok.
  6. Magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok.
  7. 80% etanol.
  8. Elution buffer (TE-bufer ili deionizovannoy voda).