DNA extraction kit from whole blood "Blood QR" Raissol for the automated station QIAcube Connect

Raissol

DNA extraction kit from whole blood "Blood QR" Raissol for the automated station QIAcube Connect is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with krov.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;DNA extraction from blood;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typeKrov

Sample Type: Krov

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. Lysis buffer - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
2. Binding buffer - dlya povysheniya sorbtsii NA na filtre kolonki;
3. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
4. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
6. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
7. Spin-kolonki - dlya filtratsii reaktsionnoy smesi i sorbtsii NA.

V nabor reagentov "GM Blood Q" variant 240R vklyucheny sleduyushchie raskhodnye materialy:
1.Odnorazovye nakonechniki 1000 uL;
2.Odnorazovye nakonechniki 200 uL;
3.Adaptery rotora dlya stantsii QIAcube.

Storage conditions

Buffers nabora "GM Blood Q" can be stored pri temperature ot +4 do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Enzyme Proteinase K must be stored pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) fermenta Proteinase K ot +4℃ do +25℃ for no more than 5 days.

Required materials and equipment

Neobkhodimye reagenty, materialy i oborudovanie dlya "GM Blood Q" variant 50 i variant 250:
  1. Ethanol 96%;
  2. Microcentrifuge tubes obemom 1,5-2 mL;
  3. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  4. Variable-volume pipettes 20-200 uL, 100-1000 uL;
  5. Odnorazovye nakonechniki dlya dozatorov peremennogo obema s aerozolnym barerom do 200 uL i 1000uL;
  6. Mernyy tsilindr obemom 100 mL s tsenoy deleniya 1 mL;
  7. Vorteks;
  8. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone ot +25 do +80ºC dlya probirok obemom 1,5-2 mL;
  9. High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min.
Neobkhodimye reagenty, materialy i oborudovanie dlya "GM Blood Q" variant 240R:
  1. Ethanol 96%;
  2. Microcentrifuge tubes obemom 1,5-2 mL;
  3. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  4. Avtomaticheskiy dozator peremennogo obema 100-1000 uL;
  5. Odnorazovye nakonechniki dlya dozatorov peremennogo obema s aerozolnym barerom do 1000uL;
  6. Mernyy tsilindr obemom 100 mL s tsenoy deleniya 1 mL;
  7. Avtomaticheskaya stantsiya QIAcube Connect (Qiagen, Germaniya);
  8. Reagent Bottles 30 ml (6 sht.) Cat. No.: 990393 (Qiagen, Germaniya).