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Reagent kit for DNA extraction from complex animal tissue samples "Tissue Strong" Raissol

Raissol
tissue_strong-50
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Reagent kit for DNA extraction from complex animal tissue samples "Tissue Strong" Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with tkani.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;Agrogenetics>>>Agrogenetics - nucleic acid extraction;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typeTkani

Sample Type: Tkani

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. Lysis buffer - na osnove detergentov i auxiliary components dlya optimal lysis;
2. Binding buffer - dlya sorbtsii NA na magnitnykh chastitsakh;
3. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
4. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Wash buffer 3 - dlya promyvki nucleic acids ot cell metabolites and salts;
6. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
7. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
8. RNase A - dlya udaleniya RNA;
9. Magnitnye chastitsy - dlya sorbtsii NA.

Instructions for use

DNA extraction procedure

Lysis
  1. V tubes obemom 1,5-2 mL place issleduemye samples.
  2. V sluchae khraneniya obraztsov v fiziologicheskom rastvore neobkhodimo tsentrifugirovat samples v techenie 5 minut pri 13 000 ob/min, posle chego polnostyu udalit supernatant. V sluchae khraneniya biomateriala v etilovom spirte samples neobkhodimo vymachivat v vode ne menee 30 minut. Zatem tsentrifugirovat samples v techenie 5 minut pri 13 000 ob/min, posle chego polnostyu udalit supernatant.
  3. Add k obraztsam 400 uL liziruyushchego bufera i 30 uL Proteinazy K, peremeshat, sbrosit kapli s pomoshchyu brief centrifugation.
  4. Incubate samples v termostate pri 60℃ ne menee 3-x chasov. Rekomenduetsya inkubirovat samples v techenie nochi dlya bolee polnogo lizisa tkani i bolshego vykhoda NA.
  5. Add k obraztsam 300 uL svyazyvayushchego bufera i tsentrifugirovat samples pri maks. ob/min v techenie 3 minut. Posle chego akkuratno perenesti maksimalnyy obem supernatanta, ne zadevaya pellet tkani, v novye tubes obemom 1,5-2 mL.
  6. Add k obraztsam 5 uL RNAazy A, akkuratno peremeshat, sbrosit kapli s pomoshchyu brief centrifugation.
  7. Incubate samples v techenie 15 minut at room temperature.
Sorbtsiya NA
  1. Add v tubes 300 uL svyazyvayushchego bufera i 100 uL magnitnykh chastits, peremeshat.
  2. Incubate samples v techenie 5 minut at room temperature, periodicheski peremeshivaya.
  3. Sbrosit kapli s pomoshchyu brief centrifugation.
  4. Incubate samples na magnitnom shtative v techenie 2 minut. Akkuratno udalit supernatant, ne zadevaya pellet magnitnykh chastits.
Nucleic acid washing
1. Add 600 uL promyvochnogo bufera 1.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
3. Incubate samples na magnitnom shtative v techenie 1 minuty, posle chego akkuratno, ne zadevaya pellet chastits, udalit supernatant.
4. Add 600 uL promyvochnogo bufera 2.
5. Repeat punkty 2-3.
6. Add 600 uL promyvochnogo bufera 3.
7. Repeat punkty 2-3.
8. Vysushit samples s otkrytymi kryshkami v termostate pri 56-60℃ ili at room temperature until complete ispareniya promyvochnogo bufera 3.
Nucleic acid elution
1. Add v tubes 50-100 uL elyuiruyushchego bufera, mix thoroughly tubes on a vortex mixer.
2. Incubate v termostate pri 60℃ v techenie 10 minut, periodicheski peremeshivaya.
3. Cbrosit kapli s pomoshchyu brief centrifugation.
4. Incubate samples na magnitnom shtative v techenie 3 minut.
5. Perenesti elyuat v novye tubes obemom 1,5-2 mL.
Optsionalno: rekomenduetsya provesti dopolnitelnoe tsentrifugirovanie elyuata na maksimalnoy skorosti dlya udaleniya ostatochnogo kolichestva magnitnykh chastits.
Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i bolee dlitelnoe vremya pri temperature -20℃.

Download the short instruction "TissueStrong" (pdf)

Download the full instruction "TissueStrong" (pdf)

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Storage conditions

Buffers nabora "Tissue Strong" can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri ponizhennykh temperaturakh khraneniya, warm the solutions to room temperature.
Magnitnye chastitsy can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. Pered applicationm suspenziyu chastits neobkhodimo tshchatelno vzbaltyvat do odnorodnogo sostoyaniya.
Enzymey Proteinase K i RNase A must be stored pri -20℃, shelf life is 12 mesyatsev.

Required materials and equipment

  1. Microcentrifuge tubes obemom 1,5-2 mL;
  2. Magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL;
  3. Variable-volume pipettes na 2-20 uL, 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
  4. Vorteks;
  5. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL;
  6. High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min.