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Reagent kit for DNA extraction from whole blood Blood M (Discontinued) Raissol

Raissol
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Reagent kit for DNA extraction from whole blood Blood M (Discontinued) Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with krov.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryDNA and RNA extraction kits;DNA extraction from blood;Agrogenetics>>>Agrogenetics - nucleic acid extraction;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typeKrov

Sample Type: Krov

Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. RBC bufer - prednaznachen dlya udaleniya eritrotsitov krovi;
2. Lysis buffer - na osnove chaotropic agents i auxiliary components dlya optimal lysis;
  1. Binding buffer - dlya sorbtsii NA na magnitnykh chastitsakh;
  2. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts;
5. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
6. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
7. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
8. Magnitnye chastitsy - dlya sorbtsii NA.

Instructions for use

DNA extraction procedure

Lysis
  1. V tubes obemom 1,5-2 mL place 300 uL krovi i add 900 uL RBC bufera.
  2. Vstryakhnut tubes on a vortex mixer.
  3. Incubate at room temperature v techenie 5-10 min.
  4. Centrifuge 1 min pri maks. ob/min (ne menee 13 tys. ob/min dlya nastolnykh tsentrifug), dalee udalit supernatant.
  5. K poluchennomu osadku kletok add 200 uL liziruyushchego bufera i 20 uL Proteinazy K, shake vigorously on a vortex mixer until complete razrusheniya konglomerata kletok.
  6. Incubate v termostate pri 56-60℃ v techenie 5 minut*, periodicheski vstryakhivaya.
  7. Sbrosit kapli s pomoshchyu brief centrifugation.
  8. Incubate at room temperature v techenie 5 minut.
Sorbtsiya NA
  1. Add k smesi 400 uL svyazyvayushchego bufera i 80 uL magnitnykh chastits.
  2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
  3. Incubate at room temperature v techenie 5 minut*, periodicheski peremeshivaya tubes.
  4. Sbrosit kapli s pomoshchyu brief centrifugation.
  5. Incubate na magnitnom shtative v techenie 5 minut.
  6. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
Nucleic acid washing
  1. Add v tubes 700 uL promyvochnogo bufera 1.
  2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation, inkubirovat na magnitnom shtative v techenie 2 minut.
  3. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
  4. Add v tubes 700 uL promyvochnogo bufera 2.
  5. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation, inkubirovat na magnitnom shtative v techenie 2 minut.
  6. Akkuratno, ne zadevaya chastitsy, udalit supernatant.
  7. Repeat punkty 4-6.
  8. Vysushit samples s otkrytymi kryshkami v termostate pri 56-60℃ v techenie 5 minut, libo at room temperature v techenie 10 minut until complete ispareniya promyvochnogo rastvora.
Nucleic acid elution
  1. Add v tubes 50-100 uL elyuiruyushchego bufera.
  2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
  3. Incubate v termostate pri 56-60℃ v techenie 10 minut, periodicheski peremeshivaya.
  4. Sbrosit kapli s pomoshchyu brief centrifugation.
  5. Incubate na magnitnom shtative v techenie 2 minut.
6. Perenesti elyuat v novye tubes obemom 1,5-2 mL.
Optsionalno: rekomenduetsya provesti dopolnitelnoe tsentrifugirovanie elyuata na maksimalnoy skorosti dlya udaleniya ostatochnogo kolichestva magnitnykh chastits.
*Uvelichenie vremeni inkubatsii sposobstvuet povysheniyu vykhoda NA na 10-30%
Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Po absorbance ratio na A260/A280 purity of the resulting solution DNA corresponds to ≥1,7.

Download the short instruction "BloodM" (pdf)

Download the full instruction "BloodM" (pdf)

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Storage conditions

Buffers nabora "GM Blood M" can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Magnitnye chastitsy can be stored pri temperature ot +4℃ do +25℃ for 12 months from the manufacturer's release date. Pered applicationm suspenziyu chastits neobkhodimo tshchatelno vzbaltyvat do odnorodnogo sostoyaniya.
Enzyme Proteinase K must be stored pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) fermenta Proteinase K ot +4℃ do +25℃ for no more than 5 days.

Required materials and equipment

  1. Microcentrifuge tubes obemom 1,5-2 mL;
  2. Rack for microcentrifuge tubes obemom 1,5-2 mL;
  3. Variable-volume pipettes na 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
  4. Vorteks;
  5. High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min;
  6. Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL;
  7. Magnitnyy shtativ dlya mikrotsentrifuzhnykh probirok obemom 1,5-2 mL.