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Reagent kit for whole-genome amplification "WGA Slassic" Raissol

Raissol
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Reagent kit for whole-genome amplification "WGA Slassic" Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with biological material.

Advantages of Raissol reagents

  • stable reagent performance and reproducible laboratory results
  • compatibility with common molecular biology protocols
  • convenient workflow for routine and research procedures
  • efficient preparation of biological material for downstream analysis

Key specifications

CategoryPCR reagents and supplies;PGT-A;Raissol
ManufacturerRaissol
Primary useDNA/RNA extraction
Sample typebiological material
Kit contents
Instructions for use
Storage conditions
Required materials and equipment

Kit contents

V nabor vklyucheny vse neobkhodimye reagenty:
1. Lysis buffer
2. R-reagent
3. Neytralizuyushchiy bufer
4. PCR-bufer
5. Polimeraza
6. Buffer dlya razbavleniya

Instructions for use

Download the short instruction "WGA Classic"

Ckachat polnuyu instruktsiyu "WGA Classic"

Provedenie protsedury polnogenomnoy amplifikatsii

Vazhno: vse protsedury dolzhny provoditsya v usloviyakh maksimalnoy sterilnosti i otsutstviya kontaminatsii, tak kak dannaya sistema imeet vysokiy uroven chuvstvitelnosti. Vse operatsii neobkhodimo provodit v osobo chistom izolirovannom laminarnom bokse. Pered nachalom protsedury sleduet provesti tshchatelnuyu dekontaminatsiyu rabochego mesta. Protsedury dolzhny provoditsya v odnorazovykh sredstvakh individualnoy zashchity.
Lysis
1. Podgotovit master-miks dlya lizisa na neobkhodimoe kolichestvo obraztsov:
1. Tshchatelno peremeshat master-miks.
2. V tubes s obraztsami obemom 0,2 mL akkuratno vnesti na stenku 6 uL master-miksa. Ne vortex mixerirovat. Obem obraztsa ne dolzhen prevyshat 5 uL.
3. Sbrosit kapli s pomoshchyu brief centrifugation.
4. Pomestit tubes v amplifikator s predvaritelno ustanovlennoy programmoy dlya lizisa posle vykhoda pribora v rabochee sostoyanie I etapa:

1. Posle ustanovki probirok v amplifikator neobkhodimo propustit I etap.
Neytralizatsiya
1. Dostat tubes iz amplifikatora. Add k smesi 3,5 uL neytralizuyushchego bufera. Ne vortex mixerirovat.
2. Sbrosit kapli s pomoshchyu brief centrifugation.
Amplifikatsiya
1. Predvaritelno ustanovit programmu dlya amplifikatsii:

Vazhno: ustanovit temperaturu kryshki amplifikatora 50°S.
Dlitelnost II etapa podbiraetsya v zavisimosti ot neobkhodimogo kolichestva produkta amplifikatsii:
2.Podgotovit master-miks dlya amplifikatsii na neobkhodimoe kolichestvo obraztsov:

3. Mix master-miks plavnym perevorachivaniem tubes, sbrosit kapli s pomoshchyu brief centrifugation.
4. Vnesti po 35,5 uL master-miksa k obraztsam.
5. Sbrosit kapli s pomoshchyu brief centrifugation.
6. Pomestit tubes v amplifikator s predvaritelno ustanovlennoy programmoy i propustit I etap.
Poluchennye produkty amplifikatsii mozhno primenyat v dalneyshikh issledovaniyakh bez dopolnitelnoy obrabotki.

Storage conditions

Kit reagentov "WGA Classic" must be stored pri temperature -20℃, shelf life is 12 mesyatsev.

Required materials and equipment

  1. Probirki v stripakh obemom 0,2 mL;
  2. Probirki obemom 1,5-2 mL;
  3. Shtativ dlya probirok obemom 0,2 mL;
  4. Shtativ dlya probirok obemom 1,5-2 mL;
  5. Variable-volume pipettes na 0,5-10 uL, 20-200 uL i sootvetstvuyushchie disposable tips;
  6. Amplifikator;
  7. Laminarnyy boks;
  8. Tsentrifuga-vstryakhivatel, tsentrifuga dlya probirok v stripakh obemom 0,2 mL.