Reagent kit for DNA extraction from seeds, flour and seedlings "SeedExt+" Raissol

Raissol

Reagent kit for DNA extraction from seeds, flour and seedlings "SeedExt+" Raissol is a Raissol reagent product for dna/rna extraction in molecular biology, genetics, diagnostic and research laboratories.

Application area

Used for sample preparation, nucleic acid analysis, PCR/qPCR workflows and sequencing-related projects where applicable.

Sample types

The product is intended for work with semena rasteniy.

Advantages of Raissol reagents

stable reagent performance and reproducible laboratory results
compatibility with common molecular biology protocols
convenient workflow for routine and research procedures
efficient preparation of biological material for downstream analysis

Key specifications

Category	DNA and RNA extraction kits;Agrogenetics>>>Agrogenetika - vydelenie iz rasteniy;Raissol
Manufacturer	Raissol
Primary use	DNA/RNA extraction
Sample type	Semena rasteniy

Sample Type: Semena rasteniy

Kit contents

Instructions for use

Storage conditions

Required materials and equipment

Kit contents

The kit includes all reagents required for DNA extraction:
1. Buffer dlya gomogenizatsii - dlya umensheniya poteri NA v protsesse gomogenizatsii v stupke;
2. Lysis buffer - na osnove detergenta i auxiliary components dlya optimal lysis;
3. Binding buffer - dlya povysheniya sorbtsii NA na filtre kolonki;
4. Wash buffer 1 - dlya promyvki nucleic acids ot cell metabolites and salts
5. Wash buffer 2 - dlya promyvki nucleic acids ot cell metabolites and salts;
6. Wash buffer 3 - dlya promyvki nucleic acids ot cell metabolites and salts;
7. Elution buffer - dlya elyutsii i khraneniya NA, pH=8,9;
8. Proteinase K - dlya bolee bystrogo i polnogo lizisa;
9. Spin-kolonki - dlya filtratsii reaktsionnoy smesi i sorbtsii NA.

Instructions for use

DNA extraction procedure

Probopodgotovka
1. Etap probopreparation obraztsa otlichaetsya v zavisimosti ot vida biomateriala:
Pri rabote s mukoy:
1.a. Vnesti v tube obemom 1,5-2 mL 50 mg naveski obraztsa, add 500 uL liziruyushchego bufera i 20 uL Proteinazy K.
Pri rabote s semenami:
1.b. Izmelchit samples do sostoyaniya muki melkogo pomola. Vnesti v tube obemom 1,5-2 mL 50 mg naveski, add 500 uL liziruyushchego bufera i 20 uL Proteinazy K.
Pri rabote s prorostkami:
1.c. Pomestit navesku 15-50 mg zelenoy chasti prorostka v tube obemom 1,5 mL i intensivno pereteret sample s pomoshchyu spetsialnogo pestika dlya 1,5 mL mikrotsentrifuzhnykh probirok do gomogennogo sostoyaniya. Add 500 uL liziruyushchego bufera. Vnesti 20 uL Proteinazy K.
1.d. Pomestit navesku 15-50 mg zelenoy chasti prorostka v farforovuyu stupku, add 500 uL liziruyushchego bufera i 500 uL bufera dlya gomogenizatsii, intensivno pereteret sample s pomoshchyu spetsialnogo pestika do gomogennogo sostoyaniya. Perenesti 500 uL gomogenata v tube obemom 1,5-2 mL. Vnesti 20 uL Proteinazy K.
Primechanie: Pri rabote na avtomaticheskom gomogenizatore trebuemaya naveska obraztsa pomeshchaetsya v spetsialnuyu 1,5-2 mL tube s keramicheskimi ili metallicheskimi businami dlya gomogenizatsii. Intensivnost programmy gomogenizatsii dolzhna byt shchadyashchaya, ne dopuskaya peregrevaniya obraztsov.
2. Intensivno peremeshat tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
Lysis
1. Incubate samples v termostate pri 56-60℃ v techenie 1-2 chasov, periodicheski vstryakhivaya.
Optsionalno: za 10 minut do okonchaniya lizisa vozmozhno ispolzovanie RNAazy A lyubogo proizvoditelya v kolichestve, rekomendovannom proizvoditelem.
2. Pomestit tubes na led ili v kholodnyy shtativ i inkubirovat v techenie 3 minut. V sluchae otsutstviya okhladitelnykh elementov inkubirovat at room temperature v techenie 10 minut until completely cooled smesi.
Sorbtsiya NA
1. Centrifuge tubes v techenie 5 minut pri 13 tys. ob/min.
2. Perenesti 300 uL supernatanta v novye tubes obemom 1,5 mL.
3. Add 300 uL svyazyvayushchego bufera, mix thoroughly tubes on a vortex mixer, sbrosit kapli s pomoshchyu brief centrifugation.
4. Perenesti ves obem smesi v spin-kolonku.
5. Centrifuge tubes v techenie 1 minuty pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
Nucleic acid washing
1. Add v tubes 650 uL promyvochnogo bufera 1.
2. Centrifuge tubes v techenie 1 minuty pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
3. Add v tubes 650 uL promyvochnogo bufera 2.
4. Centrifuge tubes v techenie 30 sekund pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
5. Add v tubes 650 uL promyvochnogo bufera 3.
6. Centrifuge tubes v techenie 30 sekund pri 13 tys. ob/min. Udalit filtrat iz sobiratelnykh probirok.
7. Repeat p 5-6.
8. Vernut kolonki v sobiratelnye tubes. Centrifuge tubes v techenie 1 minuty pri maks. ob/min.
Nucleic acid elution
1. Perenesti spin-kolonki v novye tubes obemom 1,5-2 mL. Sobiratelnye tubes utilizirovat.
2. Nanesti na membranu spin-kolonok 50-100 uL elyuiruyushchego bufera. Incubate v techenie 5 minut.
3. Centrifuge tubes v techenie 30 sekund pri maks. ob/min.
4. Utilizirovat spin-kolonki.
Optsionalno: dlya povysheniya vykhoda NA filtrat neobkhodimo povtorno nanesti na membranu kolonki i/ili ispolzovat nagretyy do 60-70℃ elyuiruyushchiy bufer.
Poluchennye rastvory nucleic acids can be stored do 7 dney pri temperature ot +2℃ do +4℃ i do dvukh let pri temperature -20℃. Po absorbance ratio na A260/A280 purity of the resulting solution DNA corresponds to ≥1,7.

Download the short instruction "SeedExt+" (pdf)

Download the full instruction "SeedExt+" (pdf)

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Storage conditions

Buffers nabora "SeedExt+" can be stored pri temperature ot +4 do +25℃ for 12 months from the manufacturer's release date. If a precipitate is present pri minimalnykh temperaturakh khraneniya, warm the solutions to room temperature.
Enzyme Proteinase K must be stored pri -20℃, shelf life is 12 mesyatsev. A short-term storage temperature increase is allowed (transportation) fermenta Proteinase K ot +4℃ do +25℃ for no more than 5 days.

Required materials and equipment

Microcentrifuge tubes obemom 1,5-2 mL;
Rack for microcentrifuge tubes obemom 1,5-2 mL;
Variable-volume pipettes na 20-200 uL, 100-1000 uL i sootvetstvuyushchie disposable tips;
Spin-kolonki s sobiratelnymi probirkami (idut v komplekte);
Vorteks;
Dry block thermostat s vozmozhnostyu podderzhaniya temperaturnogo rezhima v diapazone 25-80ºS dlya probirok obemom 1,5-2 mL;
High-speed microcentrifuge dlya probirok obemom 1,5-2 mL do 13 tys. ob/min; analiticheskie vesy s tochnostyu vzveshivaniya 0,1 mg;
Pestik dlya gomogenizatsii v 1,5 mL probirkakh;
Farforovaya stupka s pestikom.