Preparation of a target library and subsequent pair-ended sequencing of 150 bp.

Sanger sequencing is used to study a small subset of genes linked to a defined phenotype, confirm next-generation sequencing (NGS) variants, detect minor allele fractions down to 5%, or read contiguous sequences up to 1,000 bases. 

Fragment analysis is a powerful technique with simple, straightforward workflows and used in a wide-range of applications, such as detection of mutations, genotyping, identification of short tandem repeats, and gene expression profiling.

Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allow undesired amplification.
The process of designing specific primers typically involves two stages.

First, the primers flanking regions of interest are generated either manually or using software tools; then they are searched against an appropriate nucleotide sequence database using tools such as BLAST to examine the potential targets.

However, the latter is not an easy process as one needs to examine many details between primers and targets, such as the number and the positions of matched bases, the primer orientations and distance between forward and reverse primers.

The complexity of such analysis usually makes this a time-consuming and very difficult task for users, so we suggest delegating this task to our experienced specialists! 

Focused power on the MiSeq System

Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths.

Fuel your lab with NextSeq 550

The NextSeq 550 System combines tried-and-true instrument technologies and tunable output with sequencing and array capabilities. Users can run transcriptome, targeted resequencing, genotyping, and other applications all on one platform

NovaSeq 6000 Sequencing System is by far our most powerful instrument, designed to adapt to your needs so groundbreaking discoveries are always within reach. It's flexible, scalable science on your terms.
NovaSeq performs whole-genome sequencing more efficiently and cost-effectively than ever. Its scalable output generates up to 6 Tb and 20 billion reads in dual flow cell mode with simple streamlined automated workflows. Configure the system to sequence a trio in one day or up to 48 genomes in ~2 days for the most comprehensive coverage.

BGI Human Genome Sequencing Service are performed with the BGISEQ-500 sequencing system, featuring cPAS and DNA Nanoballs (DNB™) technology for
Superior data quality.
• 100bp paired-end sequencing
• Raw data, standard and customized data analysis
• Available data storage and bioinformatics applications
Sequencing Quality Standard
• Guaranteed ≥80% of bases with quality score of ≥Q30
• Raw data is qualified
Turn Around Time
• Typical 40 working days from sample QC acceptance
to filtered raw data availability
• Expedited services is available, contact your local BGI specialist for details